Analysis Note

Positive ControlHuman Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells

Application

Enzyme Immunoassay (0.1 µg/ml)r>Immunoblotting (0.5 µg/ml, colorimetric; see application references)r>Immunocytochemistry (see application references)r>Immunohistochemistry (see application references)r>Flow Cytometry (see application references)

General description

Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.

Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.

Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.

Immunogen

a recombinant protein consisting of human calreticulin fused to maltose binding protein

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.

Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)